Plant acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs) have different specificities in their forward and reverse reactions

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Lager, Ida; Lindberg Yilmaz, Jenny; Zhou, Xue-Rong; Green, Allan ORCID ID icon; Stymne, Sten


2013-12-27


Journal Article


Journal of Biological Chemistry


288


52


1


36902-36914


Acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) enzymes have central roles in acyl editing of phosphatidylcholine (PC). Plant LPCAT genes were expressed in yeast and characterized biochemically in microsomal preparations of the cells. Specificities for different acyl-CoAs were similar for seven LPCATs from five different species, with a preference for C18 unsaturated acyl-CoA and low activity with palmitoyl-CoA and ricinoleoyl (12-hydroxy-octadec-9-enoyl)-CoA. We showed that Arabidopsis LPCAT1 and LPCAT2 enzymes catalyzed the acylation and de-acylation of both sn-positions of PC, with a preference for the sn-2 position. When acyl specificities of the Arabidopsis LPCATs were measured in the reverse reaction, sn-2 bound C18 unsaturated acyl groups from PC were transferred to acyl-CoA to similar extent. However, a ricinoleoyl group at the sn-2-position of PC was removed 4-6 fold faster than an oleoyl group in the reverse reaction, despite poor utilization in the forward reaction. The data presented, taken together with earlier published reports on in vivo lipid metabolism, support the hypothesis that plant LPCAT enzymes play an important role in regulating the acyl-CoA composition in plant cells by transferring polyunsaturated and hydroxy fatty acids produced on PC directly to the acyl-CoA pool for further metabolism or catabolism.


The American Society for Biochemistry and Molecular Biology


Biochemistry and Cell Biology not elsewhere classified


https://doi.org/10.1074/jbc.M113.514398


Link to Publisher's Version


© The Authors


EP1311349


Journal article - Refereed


English


Lager, Ida; Lindberg Yilmaz, Jenny; Zhou, Xue-Rong; Green, Allan; Stymne, Sten. Plant acyl-CoA:lysophosphatidylcholine acyltransferases (LPCATs) have different specificities in their forward and reverse reactions. Journal of Biological Chemistry. 2013; 288(52 1):36902-36914. https://doi.org/10.1074/jbc.M113.514398



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