A high potency A Malaysia 97 monovalent foot-and-mouth disease vaccine protects pigs against heterologous challenge with an FMDV A SEA-97 lineage virus 4 and 7 days post vaccination

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Singanallur, Nagendra; Nguyen, H.T.T.; Fosgate, Geoffry; Morris, Jacqui; Davis, Andrew; Giles, Michelle; Kim, Phuc; Quach, Ngon; Ngo, Than Long; Tran, Hanh; Hung, V.V.; Tran, T.M; Vosloo, Wilna


2015-08-26


Journal Article


Vaccine


33


36


1


4513-4519


Pigs play a significant role during outbreaks of foot-and-mouth disease (FMD) due to their ability to amplify the virus. It is therefore essential to determine what role vaccination could play to prevent clinical disease and lower virus excretion into the environment. In this study we investigated the efficacy of the high potency double oil emulsion A Malaysia 97 vaccine (6 PD50) against heterologous challenge with an isolate belonging to the A SEA-97 lineage as soon as 4 and 7 days post vaccination (dpv). In addition, we determined whether physical separation of pigs in the same room could prevent virus transmission. Surprisingly, the vaccine protected better at 4 dpv, with no clinical disease or viral RNA detected in the blood, although three of the pigs had antibodies to the non-structural proteins (NSPs) indicating viral replication. Viral RNA was also detected in nasal and saliva swabs, but on very few occasions. Two of the pigs vaccinated seven days prior to challenge had vesicles away from the injection site, but on the inoculated foot, and two pigs had viral RNA in the blood. One pig sero-converted to the NSPs. In contrast, all unvaccinated and inoculated pigs had evidence of infection. No infection occurred in any of the susceptible pigs in the same room, but separated from the infected pigs, indicating that strict biosecurity measures were sufficient under these experimental conditions to prevent virus transmission. However, viral RNA was detected in the nasal swabs of at least one group of pigs, but apparently not at sufficient levels to cause clinical disease. Vaccination led to a significant decrease in viral RNA in vaccinated pigs compared to unvaccinated and infected pigs, even during this heterologous challenge, and could therefore be considered as a control option during outbreaks.


Elsevier


foot-and-mouth disease, serotype A, pigs, vaccination, early protection, heterologous challenge


Veterinary Virology


EP152126


Journal article - Refereed


English


Singanallur, Nagendra; Nguyen, H.T.T.; Fosgate, Geoffry; Morris, Jacqui; Davis, Andrew; Giles, Michelle; Kim, Phuc; Quach, Ngon; Ngo, Than Long; Tran, Hanh; Hung, V.V.; Tran, T.M; Vosloo, Wilna. A high potency A Malaysia 97 monovalent foot-and-mouth disease vaccine protects pigs against heterologous challenge with an FMDV A SEA-97 lineage virus 4 and 7 days post vaccination. Vaccine. 2015; 33(36 1):4513-4519. http://hdl.handle.net/102.100.100/92119?index=1



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