Dedicated industrial oilseed crops as metabolic engineering platforms for sustainable industrial feedstock production

Select | Print


Zhu, Li-Hua; Krens, Frans; Smith, Mark; Li, Xueyuan; Qi, Weicong; van Loo, Eibertus; Iven, Tim; Feussner, Ivo; Nazarenus, Tara; Huai, Dongxin; Taylor, David; Zhou, Xue-Rong; Green, Allan ORCID ID icon; Shockey, Jay; Klasson, Thomas; Mullen, Robert; Huang, Bangquan; Dyer, Jolon; Cahoon, Edgar


2016-02-26


Journal Article


Scientific Reports


6


Article 22181


Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters 20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.


Nature Publishing Group


Metabolic engineering, wax


Biological Sciences not elsewhere classified


https://doi.org/10.1038/srep22181


Link to Publisher's Version


EP163956


Journal article - Refereed


English


Zhu, Li-Hua; Krens, Frans; Smith, Mark; Li, Xueyuan; Qi, Weicong; van Loo, Eibertus; Iven, Tim; Feussner, Ivo; Nazarenus, Tara; Huai, Dongxin; Taylor, David; Zhou, Xue-Rong; Green, Allan; Shockey, Jay; Klasson, Thomas; Mullen, Robert; Huang, Bangquan; Dyer, Jolon; Cahoon, Edgar. Dedicated industrial oilseed crops as metabolic engineering platforms for sustainable industrial feedstock production. Scientific Reports. 2016; 6:Article 22181. https://doi.org/10.1038/srep22181



Loading citation data...

Citation counts
(Requires subscription to view)