Transcriptome response of Atlantic salmon (Salmo salar) to a new piscine orthomyxovirus
Samsing Pedrals, Francisca
;
Alexandre, Pamela
;
Rigby, Megan;
Taylor, Richard;
Chong, Roger;
Wynne, James
2020-10-26
Journal Article
Pathogens
9
10
Article 807
Pilchard orthomyxovirus (POMV) is an emerging pathogen of concern to the salmon industry in Australia. To explore the molecular events that underpin POMV infection, we challenged Atlantic salmon (Salmo salar) post-smolts in seawater via cohabitation. Tissue samples of head kidney and liver were collected from moribund and surviving individuals and analyzed using transcriptome sequencing. Viral loads were higher in the head kidney compared to the liver, yet liver presented more upregulated genes. Fish infected with POMV showed a strong innate immune response, that included the upregulation of pathogen recognition receptors such as RIG-I and Toll-like receptors, and induction of interferon-stimulated genes (MX, ISG15). Moribund fish also presented a dramatic induction of pro-inflammatory cytokines, contributing to severe tissue damage and morbidity. An induction of major histocompatibility complex (MHC)-class I genes (B2M) and markers of T-cell mediated immunity (CD8-alpha, CD8-beta, Perforin-1, Granzyme-A) was observed in moribund fish, but also in survivors. In addition, differential connectivity analysis showed that three key regulators (RELA/p65, PRDM1 and HLF) related to cell-mediated immunity had significant differences in connectivity in ‘healthy’ and ‘clinically affected’ or moribund fish. Collectively, our results show that T-cell mediated immunity plays a central role in the response of Atlantic salmon to the infection with POMV.
MDPI
Aquaculture
https://doi.org/10.3390/pathogens9100807
EP206407
Journal article - Refereed
English
Samsing Pedrals, Francisca; Alexandre, Pamela; Rigby, Megan; Taylor, Richard; Chong, Roger; Wynne, James. Transcriptome response of Atlantic salmon (Salmo salar) to a new piscine orthomyxovirus. Pathogens. 2020; 9(10):Article 807. https://doi.org/10.3390/pathogens9100807
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