Comparison of the alpha-amylase inhibitor-1 from common bean (Phaseolus vulgaris) varieties and transgenic expression in other legumes - post-translational modifications and immunogenicity

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Campbell, Peter; Reiner, Daniela; Moore, Andy; Lee, Rui-Yun; Epstein, Michelle; Higgins, TJ ORCID ID icon


2011-06-08


Journal Article


Journal of Agricultural and Food Chemistry


59


11


6047-6054


The seeds of peas (Pisum sativum) and chickpeas (Cicer arietinum) expressing a gene for alpha-amylase inhibitor-1 (alphaAI) from the common bean (Phaseolus vulgaris) are protected from damage by old world bruchids (pea and cowpea weevils). Here, we used electrospray ionization, time of flight mass spectrometry to compare the post-translational modifications of alphaAI from transgenic sources with the processed forms of the protein from several bean varieties. All sources showed microheterogeneity with differences in the relative abundance of particular variants due to differences in the frequency of addition of glycans, variable processing of glycans, and differences of C-terminal exopeptidase activity. The structural variation among the transgenics was generally within the range of the bean varieties. Previously mice showed allergic reactions following ingestion of transgenic pea alphaAI but not bean alphaAI. Here, only minor differences were observed following intraperitoneal sensitization. Both the transgenic pea and bean forms of alphaAI elicited Th1 and Th2 antibody isotype responses suggesting both proteins are immunogenic and could potentially be allergenic.


ACS Publications


transgenic amylase inhibitor; post-translational modification of transgenic protein; immunogenicity; glycosylation of transgenic proteins; transgenic pea; transgenic chickpea


Structural Biology (incl. Macromolecular Modelling)


https://doi.org/10.1021/jf200456j


Link to Publisher's Version


Copyright © 2011 American Chemical Society


EP106392


Journal article - Refereed


English


Campbell, Peter; Reiner, Daniela; Moore, Andy; Lee, Rui-Yun; Epstein, Michelle; Higgins, TJ. Comparison of the alpha-amylase inhibitor-1 from common bean (Phaseolus vulgaris) varieties and transgenic expression in other legumes - post-translational modifications and immunogenicity. Journal of Agricultural and Food Chemistry. 2011; 59(11):6047-6054. https://doi.org/10.1021/jf200456j



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